Selvakumar Edwardraja PhD


Peer Reviewed

  1. A universal reagent for detection of emerging diseases using bioengineered multifunctional yeast nanofragments
    Junrong Li, Christopher B Howard*, Shuvashis Dey, Kym Lowry, David M Whiley, Simon Puttick, Stephen Rose, Richard Lobb, Alain Wuethrich*,  Selvakumar Edwardraja*, Matt Trau    *
    Nature Nanotechnology 08 June 2023
    - Co-corresponding author

 

  1. Exploring performance parameters of artificial allosteric protein switches
    Cagla Ergun Ayva, Maria M Fiorito, Zhong Guo, Selvakumar Edwardraja, Joe A Kaczmarski, Dejan Gagoski, Patricia Walden, Wayne A Johnston, Colin J Jackson, Tom Nebl, Kirill Alexandrov
    Journal of Molecular Biology 14 June 2022

 

  1. Engineering and exploiting synthetic allostery of NanoLuc luciferase
    Zhong Guo, Rinky D. Parakra, Ying Xiong, Wayne A. Johnston, Patricia Walden, Selvakumar Edwardraja, Shayli Varasteh Moradi, Jacobus P. J. Ungerer, Hui-wang Ai, Jonathan J. Phillips & Kirill Alexandrov
    Nature Communications 13(789), 2022

 

  1. Generation of Nanoyeast Single-Chain Variable Fragments as High-Avidity Biomaterials for Dengue Virus Detection
    Fahimeh Farokhinejad, Rebecca E. Lane, Richard J. Lobb, Selvakumar Edwardraja, Alain Wuethrich, Christopher B. Howard, and Matt Trau
    ACS Biomater. Sci. Eng. 7(12): 5850–5860, 2021

 

  1. Connecting artificial proteolytic and electrochemical signaling systems with caged messenger peptides
    Paolo Bollella, Selvakumar Edwardraja, Zhong Guo, Claudia E Vickers, Jason Whitfield, Patricia Walden, Artem Melman, Kirill Alexandrov, Evgeny Katz
    ACS Sensors 6(10): 3596–3603, 2021

 

  1. Amplification-Free SARS-CoV-2 Detection Using Nanoyeast-scFv and Ultrasensitive Plasmonic Nanobox-Integrated Nanomixing Microassay
    Junrong Li, Alain Wuethrich, Selvakumar Edwardraja, Richard J Lobb, Simon Puttick, Stephen Rose, Christopher B Howard, Matt Trau
    Analytical Chemistry 27; 93(29): 10251–10260,  2021

 

  1. Self-powered molecule release systems activated with chemical signals processed through reconfigurable Implication or Inhibition Boolean logic gates
    Paolo Bollella, Zhong Guo, Selvakumar Edwardraja, VasanthaKrishna Kadambar, Artem Melman, Kirill Alexandrov, and Evgeny Katz
    Bioelectrochemistry 138, 107735, 2021

 

  1. Control of Allosteric Electrochemical Protein Switch Using Magnetic Signals
    Paolo Bollella, Selvakumar Edwardraja, Zhong Guo, Kirill Alexandrov, and Evgeny Katz
    ChemComm. 56(64):9206-9209, 2020

 

  1. Control of Allosteric Protein Electrochemical Switches with Biomolecular and Electronic Signals
    Paolo Bollella, Selvakumar Edwardraja, Zhong Guo, Kirill Alexandrov, and Evgeny Katz
    J. Phys. Chem. Lett.  11(14); 5549–5554, 2020

 

  1. Caged activators of artificial allosteric protein biosensors.
    Selvakumar Edwardraja, Zhong Guo, Jason Whitfield, Ignacio Retamal Lantadilla, Wayne A. Johnston, Patricia Walden, Claudia E. Vickers and Kirill Alexandrov
    ACS Synth Biol., 6:1306–1314; 2020

 

  1. Generalizable Protein Biosensors Based on Synthetic Switch Modules.
    Zhong Guo, Wayne A. Johnston, Jason Whitfield, Patricia Walden, Zhenling Cui, Elvira Wijker, Selvakumar Edwardraja, Ignacio Retamal Lantadilla, Fernanda Ely, Claudia Vickers, Jacobus P. J. Ungerer, and Kirill Alexandrov
    J. Am. Chem. Soc., 20:8128–8135; 2019

 

  1. Computational Screening of Potential Non-Immunoglobulin Scaffolds Using OCR-based Fingerprints.
    Ganapathiraman Munussami, Sriram Sokalingam, Selvakumar Edwardraja, Jung-Rae Kim, Sungwook Chung, and Sun-Gu Lee
    Korean J Chem Eng 35:717-724; 2018

 

  1. Rational Design of an Anticalin-Type Sugar-Binding Protein Using a Genetically Encoded Boronate Side Chain.
    Selvakumar Edwardraja, Andreas Eichinger, Ina Theobald, Carina Andrea Sommer, Andreas J. Reichert, and Arne Skerra
     ACS Synthetic Biology 6(12):2241-2247; 2017
    Crystal Structure: (PDB ID: 5MHH)

 

  1. Biochemical and spectroscopic characterization of dinuclear Mn-sites in artificial four-helix bundle proteins.
    Tien Olson, Eduardo E, Selvakumar Edwardraja, Elizabeth C, Marco F, JoAnn W, Giovanna Ghirlanda, and James P. Allen
         BBA - Bioenergetics 1858(12):945-954; 2017

 

  1. Generation of efficient fingerprint for GFP-like fold and computational identification of potential GFP-like homologs.
    Selvakumar Edwardraja, Ganapathiraman Munussami, Amit Goyal and Sun-Gu Lee
    Biotechnology and Bioprocess Engineering     21:712-19; 2016

 

  1. Design of Dinuclear Manganese Cofactors for Bacterial Reaction Centers.
    Tien Olson, Eduardo E, Selvakumar Edwardraja, Chad RS, Williams J, Giovanna Ghirlanda, and James P. Allen
    BBA Bioenergetics     1857(5):539-47; 2016
    Crystal Structure: (PDB ID: 5C39)

    Special Issue entitled Biodesign for Bioenergetics

 

  1. The Copper Environment in Artificial Metalloproteins Probed by EPR Spectroscopy.
    Marco Flores, Tien Olson, Dong Wang, Selvakumar Edwardraja, Sandip Shinde, JoAnn C. Williams, Giovanna Ghirlanda, and James P. Allen
    Journal of Physical Chemistry B 119(43):13825-13833; 2015

    Special Issue in Honor of Professor Wolfgang Lubitz

 

  1. Targeted Delivery of Ubiquitin-Conjugated BH3 Peptide-Based Mcl-1 Inhibitors into Cancer Cells.
    Avinash Muppidi, Kenichiro Doi, Selvakumar Edwardraja, Surya V. S. R. K. Pulavarti, Thomas Szyperski, Hong-Gang Wang, Qing Lin
    Bioconjugate Chemistry 25(2):424-432; 2014

 

  1. 3-hydroxyisobutyrate dehydrogenase-I from Pseudomonas denitrificans ATCC 13867 degrades 3-hydroxypropionic acid
    Philah Lee, Subramanian Mohan Raj, Shengfang Zhou, Somasundar Ashok, Selvakumar Edwardraja, Sunghoon Park
    Biotechnology and Bioprocess Engineering, 19(1):1-7; 2014

 

  1. Cloning, Expression and Characterization of 3-Hydroxyisobutyrate Dehydrogenase from Pseudomonas denitrificans ATCC 13867.
    Shengfang Zhou, Subramanian Mohan Raj, Somasundar Ashok, Selvakumar Edwardraja, Sun-Gu Lee, Sunghoon Park
    PLOS ONE 8(5): e62666, 2013

 

  1. Generation of anti-c-Met single domain antibody fragment based on human stable frameworks.
    Selvakumar Edwardraja, Sokalingam Sriram, Raghunathan Govindan, Bum-Yeol Hwang, Sun-Gu Lee
    Biotechnology and Bioprocess Engineering, 17(6):1120-1127; 2012

 

  1. Rational Design of Proteolytically Stable, Cell-Permeable Peptide-Based Selective Mcl-1 Inhibitors.
    Avinash Muppidi, Kenichiro Doi, Selvakumar Edwardraja, Eric Drake, Andrew M. Gulick, Hong-Gang Wang, Qing Lin
    J. Am. Chem. Soc., 134(36): 14734-14737; 2012
    Crystal Structure: (PDB ID: 4G35)

 

  1. Copper-Free Sonogashira Cross-Coupling for Functionalization of Alkyne-Encoded Proteins in Aqueous Medium and in Bacterial Cells.
    Nan Li, Reyna K. V. Lim, Selvakumar Edwardraja, Qing Lin
    ChemInform, 43(13); 2012

 

  1. Enhancing the productivity of soluble green fluorescent protein through methionine-residue specific consensus approach
    Nagasundarapandian Soundrarajan, Selvakumar Edwardraja, Sun-Gu Lee, Hyungdon Yun, Niraikulam Ayyadurai
    African Journal of Biotech, 11(5):1059-1064; 2012

 

  1. Copper-Free Sonogashira Cross-Coupling for Functionalization of Alkyne-Encoded Proteins in Aqueous Medium and in Bacterial Cells
    Nan Li, Reyna K. V. Lim, Selvakumar Edwardraja, Qing Lin
    J. Am. Chem. Soc., 133(39):15316–15319; 2011

 

  1. Enhancing the thermal stability of a single-chain Fv fragment by in vivo global fluorination of the proline residues
    Selvakumar Edwardraja, Sokalingam Sriram, Raghunathan Govindan, Nediljko Budisa, Sun-Gu Lee
    Molecular BioSystems, 7(1):258-265; 2011

    (PMDB ID: PM0078682 - 4R-FP-hu-MscFv-model.pdb)

 

  1. Redesigning of Anti-c-Met Single Chain Fv Antibody for the Cytoplasmic Folding and Its Structural Analysis
    Selvakumar Edwardraja, Rameshkumar Neelamegam, Vijayaraj, Subramanian Venkatesan, Sun-Gu Lee
    Biotechnology and Bioengineering, 106(3):367-375; 2010

    (PMDB ID: PM0076146 - wt-MscFv-model-kabat.pdb)
     (PMDB ID: PM0076147 - hu-MscFv-model-kabat.pdb)


  1. In vivo Production of Functional Single-Chain Fv Fragment with an N-Terminal-Specific Bio-orthogonal Reactive Group
         Selvakumar Edwardraja, Neelamegam Rameshkumar, Sun-Gu Lee, Soo-Jae Lee, Hyung-Soon Park
    ChemBioChem, 11(4):498-501; 2010


  1. A Novel NAD+-Dependent Aldehyde Dehydrogenase Encoded by the puuC gene of Klebsiella pneumoniae DSM 2026 Utilizes 3-Hydroxypropionaldehyde as a Substrate
    Subramanian Mohan Raj, Chelladurai Rathnasingh, Woo-Chel, Selvakumar Edwardraja, Sunghoon Park
    Biotechnology and Bioprocess Engineering, 15(1):131-138; 2010


  1. Importance of Expression System in the Production of Unnatural Recombinant Proteins in Escherichia Coli
    Niraikulam Ayyadurai, Rameshkumar Neelamegam, Soundrarajan Nagasundarapandian, Selvakumar Edwardraja, Hyung Soon Park, Soo Jae Lee, Tae Hyeon Yoo, Hyungdon Yoon, Sun-Gu Lee
    Biotechnology and Bioprocess Engineering, 14(3):257-265; 2009


  1. Cloning, expression, and characterization of an aldehyde dehydrogenase from Escherichia coli K-12 that utilizes 3-Hydroxypropionaldehyde as a substrate
    Ji-Eun Jo, Subramanian Mohan Raj, Chelladurai Rathnasingh, Selvakumar Edwardraja, Woo-Chel Jung, Sunghoon Park
    Applied Microbiology and Biotechnology 81(1): 51-60; 2008


  1. Improving the Productivity of Single-Chain Fv Antibody Against c-Met by Rearranging the Order of its Variable Domains.
    Yu-Jin Kim, Rameshkumar Neelamegam, Mi-Ae Heo, Selvakumar Edwardraja, Hyun-Jong Paik, Sun-Gu Lee
    Journal of Microbiology and Biotechnology 18(6):1186-90; 2008


Conference Papers (International)

  • 2017 KSBB Fall Meeting and International Symposium, 2017.10, 251-251
    Identification of Potential Cystatin and Lipocalin Proteins Using OCR-based Fingerprint
    Ganapathiraman MUNUSSAMI, Sriram SOKALINGAM, Selvakumar EDWARDRAJA, Sun-Gu LEE

 

  • Gordon Conference on Photosynthesis, Waltham, MA, USA; Jun/2015
    Design of dinuclear manganese cofactors for bacterial reaction centers, James P. Allen, Tien L. Olson, Eduardo Espiritu, Marco Flores, Chad Simmons, Selvakumar Edwardraja, Giovanna Ghirlanda, and JoAnn C. Williams

 

  • 16th International Congress on Photosynthesis Research, St. Louis, MO, USA; Aug/2013
    Enzymatic activity of a Mn-binding peptide, T. L. Olson, S. Edwardraja, M. Flores, G. Ghirlanda, and J. P. Allen

 

  • Gordon Conference: Photosynthesis, Davidson, NC, USA; Jul/2012
    Designing metal binding peptides to catalyze water oxidation, Tien Olson, Selvakumar Edwardraja, Dong Wang, Shandip Shinde, Marco Flores, Giovanna Ghirlanda, and James P. Allen (POSTER)

 

  • Antibody Engineering & Antibody Therapeutics, San Diego, CA, USA; Dec/2009
    Generation of fluorinated scFv and investigation on its stability, Sriram Sokalingam, Govindan Raghunathan, Selvakumar Edwardraja and Sun-Gu Lee (POSTER)

 

  • Enzyme Engineering XX, Groningen, Netherlands; Sep/2009
    Engineering of GFP through combinatorial mutagenesis of methionine residues based on consensus sequence, Niraikulam Ayyadurai, Selvakumar Edwardraja, Soundrarajan Nagasundarapandian, and Sun-Gu Lee (POSTER)

 

  • GRC Polymer Physics, Newport, RI, USA; Jun/2008
    Engineering the activity/fodability of alkynylated Green Fluorescent Protein, Niraikulam Ayyadurai, Edwardraja Selvakumar, Soundrarajan Nagasundarapandian, Sun-Gu Lee (POSTER)

 

  • Peptides, Chemistry & Biology Of, Ventura, CA USA; Feb/2008
    Engineering of anti-c-Met scFv for high expression and efficient folding in the cytoplasm of Escherichia coli, Edwardraja Selvakumar and Sun-Gu Lee (POSTER)

 

  • 1st International Conference on Biomolecular Engineering, Coronado Island, CA, USA; Jan/2007 (Attended)

 

  • 2010 Fall KSBB Meeting and International Symposium, SONGDO, Incheon, Korea; Oct/2010
    CO Induced Hydrogenase Gene in Citrobacter amalonaticus Y19 : An Effect of Adaptive Evolution? Gurpreet Kaur, Selvkumar Edwardraja, Jung Eun Lee, Yang Seon Kim, Eun Joung Moon and Sunghoon Park

 

  • 2009 Fall KSBB Meeting and International Symposium, DCC, Daejeon, Korea; Nov/2009
    In vivo based N-terminal specific addition of bio-orthogonal reactive group on single-chain Fv fragment Selvakumar Edwardraja, and Sun-Gu Lee (*Awarded for Best Oral Presentation)

 

  • 2008 Fall KSBB Meeting and International Symposium, ICC Jeju, Korea; Oct/2008
    Structure-function-stability relationship of anti-c-Met single chain Fv antibody – a structural insight Selvakumar Edwardraja, and Sun-Gu Lee (Oral)

 

  • 2008 Fall KSBB Meeting and International Symposium, ICC Jeju, Korea; Oct/2008
    In vivo incorporation of alkynyl group into the intracellular chimeric scFv against c-Met
    Rameshkumar Neelamegam, Selvakumar Edwardraja, and Sun-Gu Lee (Awarded for Best Poster)

 

  • 2008 Fall KSBB Meeting and International Symposium, ICC Jeju, Korea; Oct/2008
    Engineering the Solubility of Green Fluorescence Protein for the Incorporation of Methionine Analogues Niraikulam Ayyadurai, Selvakumar Edwardraja, Soundrarajan Nagasundarapandian, and Sun-Gu Lee (Poster)

 

  • 2007 KSBB (Korean Society of Biotechnology and Bioengineering) Fall Meeting, Dae-Gu, Korea; Oct/2007
    Improving the productivity of functional scFv against c-Met by rearranging the order of variable domains and chaperone co-expression
    Yu-Jin Kim, Mi-Ae Heo, Rameshkumar Neelamegam, Selvakumar Edwardraja, Sun-Gu Lee (Poster)

 

Conference Papers (Domestic)

  • The National Academy of Sciences, India 75th Annual Session, Pondicherry, India; Dec/2005
    Prediction of Protein-Protein Interactions in Deinococcus radiodurans R1 strain using computational methods
    Selvakumar E., Shanthi N., Sameer Saurava Prusty, Ezhil Maran K. and Komala G (Oral)

 

Citation Metrics (Researcher ID)

Total Articles in Publication List: 17
Articles With Citation Data: 15
Sum of the Times Cited: 152
Average Citations per Article: 11.69
h-index: 6
Last Updated: 16/Nov/2014